egfp actin Search Results


pgtag 2a egfp b actin plasmid  (Addgene inc)
93
Addgene inc pgtag 2a egfp b actin plasmid
Pgtag 2a Egfp B Actin Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgtag 2a egfp b actin plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
pgtag 2a egfp b actin plasmid - by Bioz Stars, 2026-02
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actin gfp  (Addgene inc)
93
Addgene inc actin gfp
Actin Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/actin gfp/product/Addgene inc
Average 93 stars, based on 1 article reviews
actin gfp - by Bioz Stars, 2026-02
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mice expressing egfp under the control of the chicken actin promoter (actb.gfp)  (Jackson Laboratory)
90
Jackson Laboratory mice expressing egfp under the control of the chicken actin promoter (actb.gfp)
Mice Expressing Egfp Under The Control Of The Chicken Actin Promoter (Actb.Gfp), supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mice expressing egfp under the control of the chicken actin promoter (actb.gfp) - by Bioz Stars, 2026-02
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mouse β-actin p38 iκb egfp antibody  (Rockland Immunochemicals)
90
Rockland Immunochemicals mouse β-actin p38 iκb egfp antibody
Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the <t>eGFP</t> vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
Mouse β Actin P38 Iκb Egfp Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse β-actin p38 iκb egfp antibody/product/Rockland Immunochemicals
Average 90 stars, based on 1 article reviews
mouse β-actin p38 iκb egfp antibody - by Bioz Stars, 2026-02
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transgenic mice expressing egfp under the control of the β-actin promoter β-actin-egfp  (Jackson Laboratory)
90
Jackson Laboratory transgenic mice expressing egfp under the control of the β-actin promoter β-actin-egfp
Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the <t>eGFP</t> vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
Transgenic Mice Expressing Egfp Under The Control Of The β Actin Promoter β Actin Egfp, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transgenic mice expressing egfp under the control of the β-actin promoter β-actin-egfp/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
transgenic mice expressing egfp under the control of the β-actin promoter β-actin-egfp - by Bioz Stars, 2026-02
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egfp controlled β-actin promotor  (Cyagen Biosciences)
90
Cyagen Biosciences egfp controlled β-actin promotor
Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the <t>eGFP</t> vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
Egfp Controlled β Actin Promotor, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egfp controlled β-actin promotor/product/Cyagen Biosciences
Average 90 stars, based on 1 article reviews
egfp controlled β-actin promotor - by Bioz Stars, 2026-02
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flex-β-actin- egfp mice  (Jackson Laboratory)
90
Jackson Laboratory flex-β-actin- egfp mice
Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the <t>eGFP</t> vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
Flex β Actin Egfp Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flex-β-actin- egfp mice/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
flex-β-actin- egfp mice - by Bioz Stars, 2026-02
90/100 stars
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lzrs-egfp-actin-ires-zeocin  (Sanquin)
90
Sanquin lzrs-egfp-actin-ires-zeocin
Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the <t>eGFP</t> vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
Lzrs Egfp Actin Ires Zeocin, supplied by Sanquin, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lzrs-egfp-actin-ires-zeocin/product/Sanquin
Average 90 stars, based on 1 article reviews
lzrs-egfp-actin-ires-zeocin - by Bioz Stars, 2026-02
90/100 stars
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ß-actin-egfp mice  (Charles River Laboratories)
90
Charles River Laboratories ß-actin-egfp mice
Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the <t>eGFP</t> vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
ß Actin Egfp Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ß-actin-egfp mice/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
ß-actin-egfp mice - by Bioz Stars, 2026-02
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antibody against bcov nsp1, nucleocapsid (n) protein, or β-actin  (Jackson Laboratory)
90
Jackson Laboratory antibody against bcov nsp1, nucleocapsid (n) protein, or β-actin
Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the <t>eGFP</t> vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
Antibody Against Bcov Nsp1, Nucleocapsid (N) Protein, Or β Actin, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody against bcov nsp1, nucleocapsid (n) protein, or β-actin/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
antibody against bcov nsp1, nucleocapsid (n) protein, or β-actin - by Bioz Stars, 2026-02
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egfp-actin and eyfp-tubulin  (Qiagen)
90
Qiagen egfp-actin and eyfp-tubulin
Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the <t>eGFP</t> vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
Egfp Actin And Eyfp Tubulin, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egfp-actin and eyfp-tubulin/product/Qiagen
Average 90 stars, based on 1 article reviews
egfp-actin and eyfp-tubulin - by Bioz Stars, 2026-02
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cnp-enhanced green fluorescent protein (egfp) and β-actin-gfp transgenic mice (tg actbgfp)  (Jackson Laboratory)
90
Jackson Laboratory cnp-enhanced green fluorescent protein (egfp) and β-actin-gfp transgenic mice (tg actbgfp)
Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the <t>eGFP</t> vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).
Cnp Enhanced Green Fluorescent Protein (Egfp) And β Actin Gfp Transgenic Mice (Tg Actbgfp), supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cnp-enhanced green fluorescent protein (egfp) and β-actin-gfp transgenic mice (tg actbgfp)/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
cnp-enhanced green fluorescent protein (egfp) and β-actin-gfp transgenic mice (tg actbgfp) - by Bioz Stars, 2026-02
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Image Search Results


Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the eGFP vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).

Journal: International Journal of Molecular Sciences

Article Title: Prolonged Inhibition of the MEK1/2-ERK Signaling Axis Primes Interleukin-1 Beta Expression through Histone 3 Lysine 9 Demethylation in Murine Macrophages

doi: 10.3390/ijms241914428

Figure Lengend Snippet: Priming RAW264.7 cells with U0126 is partly mediated by repressing genes involved in H3K9 methylation. ( A ) Based on the transcriptomic analysis, the expression of epigenetic-related genes with changes greater than 2-fold in U0126-primed cells are grouped and plotted with bubble heat maps. ( B ) Expressions of Cbx5 , Suv39h1 , and Kdm7a were confirmed via qPCR in U0126-primed and non-primed cells. Statistical significance was determined using multiple unpaired two-tailed t-tests ( n = 3). ( C , D ) Cells were treated with various inhibitors for 18 h and then activated by LPS (100 ng/mL) for 6 h. Expression of IL-1β was quantified via qPCR. ( C ). Cells were treated with U0126 together with or without KDM2/7 and/or KDM5 inhibitors. ( D ) Cells were treated with inhibitors against the H3K9 methyltransferases G9a (BIX01294, 1.5 µM) and/or SUV(VAR)3–9 (chaetocin, 100 nM) or the DNMT inhibitors 5-azacytidine (2 µM) and SGI-1027 (10 µM). ( E ) Cells were stably transfected with the eGFP vector control (Vector) or the GFP-mCBX5 plasmid (CBX5). Left panel: Stable transfections were confirmed via eGFP or eGFP-CBX5 Western blots, using p38 antibody as a loading control. Right panel: Cells were activated with LPS (100 ng/mL) for 6 h, and IL-1β expression was measured with qPCR. Statistical significance was determined using one-way ANOVA tests followed by Dunnett’s multiple comparisons test ( n = 3; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).

Article Snippet: The primary antibodies for mouse β-actin, including p38, IκB, and eGFP, were purchased from Rockland, Bridgeport, NJ, USA (#600-401-886; 1:2000 dilution), Cell Signaling Technologies, Danvers, MA, USA (#9212, #4814; 1:2000 dilution), and Clontech Lab., Mountain View, CA, USA (#632381; 1:1000 dilution), respectively; the secondary antibodies for rabbit IgG-HRP and mouse IgG-HRP were from Santa Cruz, Dallas, TX, USA (#SC-2357) and Thermo Scientific, Waltham, MA, USA (#32430), respectively.

Techniques: Methylation, Expressing, Two Tailed Test, Stable Transfection, Transfection, Plasmid Preparation, Control, Western Blot